Research Related Activity
Faculties are involved in interdisciplinary research projects.
Currently a BRNS-DAE sponsored major research project "Spatial Distribution of Uranium and associated water quality parameters in groundwater/ drinking water of five districts (Aurangabad, Beed, Buldhana, Parbhani and Jalna) of Maharashtra"is run jointly with department of Environment ScienceDr.Babasaheb Ambedkar Marathwada University.The department is actively engaged in finding the economically pharmacologically important ingredients from the plant sources.
- Understanding Multi-drug resistance in bacteria
- Enzyme biology
- Environmental Biotechnology
- Applications of microwaves in biotechnology
- Molecular Microbiology
Selected Research Article
ANTIFUNGAL AND ANTICANCER POTENTIAL OF ARGEMONE MEXICANAL.
Author - Nilesh V. More and Arun S. Kharat ,*
Abstract - Medicinal plants are widely used to treat infectious diseases, metabolic disorders and cancer. Argemonemexicana L. (A. mexicana), commonly found on desolate land of Marathwada (Maharashtra, India) has been used to treat oral cavity infections. In this study, cold aqueous and methanolic extracts were prepared from A. mexicana stem and leaves. These extracts were tested for their antifungal and anticancer activities. The antifungal activity was tested using the agar well diffusion method, while the anticancer activity against immortalized cell lines was assessed by trypan blue assay.
OPTIMIZATION OF DISC DIFFUSION ASSAY TO STUDY PECTINASE ACTIVITY IN AGAR GEL
Author - Jeetendra A. Kulkarni1, Mangesh N. Kothari, and Mirza M. VaseemBaig*
Abstract - The activity pattern of Pectinase in gel matrix was studied. The behaviour of hydrolytic enzyme in gels as comparedto that of its behaviour in solution is quite different. The study of this behaviour is required to better understand theenzyme action in nature. Each enzyme is quite specific in acting on a particular substrate to produce particularproduct. This specificity can be exploited for the application of enzymes. Present study was designed to understandthe diffusion of enzymes in gels, enzyme substrate reaction in gel, enzyme parameter optimization and to determinethe effect of substrate on enzyme activity in Agar gel.The optimum pH and temperature were found to be 3 and 600C respectively.
OPTIMIZATION OF CELLULASE PRODUCTION FOR BACILLUS SP. AND PSEUDOMONAS SP. SOIL ISOLATES.
Author - Arusha P. Nandimath, Kiran R. Kharat , Shanti G. Guptaand Arun S. Kharat*>
Abstract - This investigation deals with optimizing the cellulase producing bacterial isolates for better enzyme yield. Cellulose degrading bacteria were isolated from garden soil (Aurangabad, MS, India). Amongst 40 different bacterial isolates, two of the bacterial isolates, 2b and 38b, were processed for preliminary identification with morphological, cultural and biochemical characterization. Based on these studies, strain 2b was qualified to belong to Pseudomonas sp. and strain 38b was qualified to belong to Bacillus sp. Optimization of the fermentation medium for maximum cellulase production was carried out for both strains of 2b and 38b.
Project Name / Scheme
"Spatial Distribution of Uranium and associated water quality parameters in groundwater/ drinking water of five districts (Aurangabad, Beed, Buldhana, Parbhani and Jalna) of Maharashtra"
Faculty from this department ( JeetendraKulkakarni ) is co-investigator in this project
the investigation is related to screening of water resources in the five districts of Marathwada with respect to occurrence and implications of radioactivity